caspase 1 antibody  (Boster Bio)

Journal: Oncology Letters

Article Title: Transferrin receptor serves a role in promoting PANoptosis in thyroid cancer

doi: 10.3892/ol.2025.15394

Figure Lengend Snippet: TFRC contributes to PANoptosis in TC cells. Effects of (A) PGMLV-CMV-MCS-EF1-ZsGreen1-T2A-Puro and (B) shRNA transfection on the expression of TFRC mRNA in BCPAP and K1 cells, assessed using reverse transcription-quantitative PCR assay. (C) PI/Calcein-AM staining was performed to assess the effect of TFRC on cell death in BCPAP (OV-NC and OV-TFRC groups) and K1 (KD-NC and KD-TFRC groups) cells. PI (red) and Calcein-AM (green) label dead and viable cells, respectively. (D) YO-PRO-1/PI staining was performed to evaluate pyroptosis, apoptosis and necroptosis in BCPAP and K1 cells following exogenous modulation of TFRC expression. YO-PRO-1 (green) indicates apoptotic or necroptotic cells, whereas PI (red) labels necroptotic or pyroptotic cells. (E) Western blot analysis of markers associated with pyroptosis (cleaved-CASP1/CASP1), apoptosis (cleaved-CASP3/CASP3) and necroptosis (pMLKL/tMLKL and tRIPK3), with corresponding quantitative analyses. TFRC, transferrin receptor; sh, short hairpin; OV, overexpression; NC, negative control; KD, knockdown; CASP; caspase; MLKL, mixed lineage kinase domain like pseudokinase; pMLKL, phospho-MLKL; tMLKL, total-MLKL; tRIPK3, total receptor interacting serine/threonine kinase 3.

Article Snippet: Membranes were blocked with 5% skimmed milk at room temperature for 1 h and incubated with the following primary antibodies overnight at 4°C: Vinculin mouse mAb (1:50,000; cat. no. 66305-1-Ig; Proteintech Group, Inc.), CASP3 mouse mAb (1:2,000; cat. no. bsm-33284M; BIOSS), cleaved-CASP3 mouse mAb (1:2,000; cat. no. bsm-33199M; BIOSS), CASP1 rabbit pAb (1:2,000; cat. no. 22915-1-AP; Proteintech Group, Inc.), cleaved-CASP1 rabbit pAb (1:3,000; cat. no. PA5-77886; Thermo Fisher Scientific, Inc.), phospho-(p)MLKL rabbit pAb (1:2,000; cat. no. PA5-105677; Thermo Fisher Scientific, Inc.), total-(t)MLKL mouse mAb (1:50,000; cat. no. 66675-1-Ig; Proteintech Group, Inc.) and tRIPK3 rabbit pAb (1:10,000; cat. no. 29080-1-AP; Proteintech Group, Inc.).

Techniques: shRNA, Transfection, Expressing, Reverse Transcription, Real-time Polymerase Chain Reaction, Staining, Western Blot, Over Expression, Negative Control, Knockdown

Journal: Cancer Cell International

Article Title: Natural compound triptolide induces caspase-3/GSDME-mediated pyroptosis by promoting ROS accumulation in small cell lung cancer

doi: 10.1186/s12935-025-04071-8

Figure Lengend Snippet: CASP3 is the upstream modulator of GSDME-mediated pyroptosis upon TPL treatment. (A-B) The protein levels of the CASP3 and CASP1 after TPL treatment (12, 24, and 48 h), measured by Western blot analysis. Ctrl, control; CASP3, caspase-3; C-CASP3, cleaved caspase-3. (C and D) Morphological changes and relative LDH activity at 48 h following TPL treatment combined with CASP3 inhibition by Z-DEVD-FMK, red arrows: pyroptotic cells. (E–G) The protein levels of GSDME (F: full length; N N-terminal fragment) and CASP3 at 48 h following TPL treatment combined with CASP3 inhibition by Z-DEVD-FMK. Data are presented as mean ± SD ( n = 3). * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; unpaired t-test

Article Snippet: Following a 1 h blockade with 5% BSA, the membranes were incubated overnight at 4 °C with primary antibodies against GSDME (Abcam, ab215191), N-terminal fragment of GSDME (GSDME-N) (Abcam, ab222407), GSDMD (Abcam, ab219800), CASP3 (Santa Cruz, sc-56046), cleaved CASP3 (CST, 9661), CASP1 (Proteintech, 22915-1-AP), GAPDH (Proteintech, 60004-1-Ig), and α-tubulin (Proteintech, 1124-1-AP).

Techniques: Western Blot, Control, Activity Assay, Inhibition